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In the brain, observed pathologic changes include congested blood vessels, perivascular cavitation, pericellular halos, vacuolation of neuropils, darkly stained nuclei, pyknotic nuclei amid associated vacuolation of the neuropil, and acute eosinophilic necrosis

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coli mutants Bacterial colonies from individual bacterial mutants were streaked on LB agar plates, grown at 37 °C, and genotyped by PCR using the kanamycin-cassette-specific primers (forward primer 5’-CGGTGCCCTGAATGAACTGC-3’, reverse primer 5’-CAGTCATAGCCGAATAGCCT-3’) and genomic primers for individual bacterial mutants, which were designed at starting 100-300 bases upstream of start-codon (forward primer) or downstream of stop codon (reverse primer)