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Subsequently, the homogenate was centrifuged at 800×g for 15 min and the levels of MDA, SOD, and CAT in each group were individually measured by commercial assay kits according to the manufacturer's instructions

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In addition, if melatonin is used in combination with hypothermia, the neuroprotective effects are greater than hypothermia alone 134,141,142,143,144,145,146